Biological magnification is defined as the phenomenon of accumulation or increase in the concentration of certain toxic substances at each trophic level.Some harmful or toxic substances, such as: DDT sprinkled to kill pests in food plants, have been found to be washed down into soil or water bodies. Plants, along with water and minerals, absorb these chemicals from the soil, while aquatic plants and animals take these chemicals from the water bodies.Toxic substances enter our body when we eat these plants or aquatic plants and animals.Yes, the magnification at different levels of the ecosystem is different. Animals at higher trophic levels take in more amount of these toxic substances along with their food than animals at lower trophic levels. As the level of the food chain increases, the magnification also increases.